This study was aimed at preliminarily assessing the cytoprotective and antioxidative effects of rice bran extracts (RBEs) from\na Sarawak local rice variety (local name: ââ?¬Å?BJLNââ?¬Â) and a commercial rice variety, ââ?¬Å?MR219,ââ?¬Â on oxidative stress in rat H9c2(2-\n1) cardiomyocytes. The cardiomyocytes were incubated with different concentrations of RBE and hydrogen peroxide (H2O2),\nrespectively, to identify their respective IC50 values and safe dose ranges. Two nonlethal and close-to-IC50 doses of RBE were\nselected to evaluate their respective effects on H2O2 induced oxidative stress in cardiomyocytes. Both RBEs showed dosedependent\ncytotoxicity effects on cardiomyocytes. H2O2 induction of cardiomyocytes pretreated with RBE further revealed the\ndose-dependent cytoprotective and antioxidative effects of RBE via an increase in IC50 values of H2O2. Preliminary analyses of\ninduction effects of RBE and H2O2 on cellular antioxidant enzyme, catalase (CAT), also revealed their potential in regulating\nthese activities and expression profile of related gene on oxidative stress in cardiomyocytes. Pretreated cardiomyocytes significantly\nupregulated the enzymatic activity and expression level of CAT under the exposure of H2O2 induced oxidative stress. This\npreliminary study has demonstrated the potential antioxidant effects of RBE in alleviating H2O2-mediated oxidative injuries via\nupregulation in enzymatic activities and expression levels of CAT.
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